Labsystems Enzyme Immunoassay for Chlamydia pneumoniae Also Detects Chlamydia psittaci Infections
نویسندگان
چکیده
منابع مشابه
Labsystems enzyme immunoassay for Chlamydia pneumoniae also detects Chlamydia psittaci infections.
In a recent article, Bas and collaborators compared different serological methods to detect chlamydial antibodies in patients with Chlamydia trachomatis infections and healthy blood donors (1). Using the Labsystems enzyme immunoassay (EIA) for C. pneumoniae, no cross-reaction between C. trachomatis and C. pneumoniae was found. However, Gnarpe and collaborators recently showed cross-reactions be...
متن کاملRapid detection of Chlamydia pneumoniae by PCR-enzyme immunoassay.
Chlamydia pneumoniae is an important human respiratory pathogen. Laboratory diagnosis of infection with this organism is difficult. To facilitate the detection of C. pneumoniae by PCR, an enzyme immunoassay (EIA) for analysis of PCR products was developed. Biotin-labeled PCR products generated from the 16S rRNA gene of C. pneumoniae were hybridized to a digoxigenin-labeled probe and then immobi...
متن کاملChlamydia trachomatis and Chlamydia pneumoniae infections in children and adolescents.
2004;25;43 Pediatrics in Review Margaret R. Hammerschlag Adolescents Chlamydia trachomatis and Chlamydia pneumoniae Infections in Children and http://pedsinreview.aappublications.org/content/25/2/43 located on the World Wide Web at: The online version of this article, along with updated information and services, is http://pedsinreview.aappublications.org/content/suppl/2005/01/26/25.2.43.DC1.htm...
متن کاملMultiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples.
AIMS To develop a multiplex polymerase chain reaction (PCR) for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples. METHODS Oligonucleotide primers for the amplification of the DNA of these three organisms were optimised for use in combination in the same reaction. PCR products were detected by hybridisation with pooled int...
متن کاملDetection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR.
AIMS To use the polymerase chain reaction (PCR) to detect Chlamydia pneumoniae and Chlamydia psittaci in sputum samples. METHODS A nested PCR was developed, the first stage of which amplified DNA from both C pneumoniae and C psittaci while the second stage targeted specifically at C pneumoniae, allowing the two species to be differentiated. The primers were designed not to amplify sequences f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2001
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.39.9.3425-3426.2001